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Friday, January 30, 2013Structural and functional characterization of CaMV P35 promoter.
CaMV 35S promoter (35SP) is one of the well-characterized promoters and widely used to drive foreign gene expression. In the present study, we characterized a 35SP from cauliflower mosaic virus (CaMV) and analyzed its tissue-specific and light-inducible expression in Nicotiana benthamiana. The CaMV 35SP promoter sequence is located within the 5′ flanking region of the 35S gene and drives expression of the downstream reporters. Cloning and transient expression of mutated 35SP, its deletion, and fusion to 35SP suggest that the 35SP contains three functional domains: a TATA box, a hormone responsive element (HRE) and a light responsive element (LRE). Promoters that lacked the HRE and LRE resulted in a specific expression pattern in N. benthamiana leaf epidermal cells, indicating that these two regulatory elements function in the promoter. Under high light intensity conditions, overexpression of HY5 protein, which is a key factor in the photoinduction of LRE-lacking promoters, significantly promoted the expression of the promoter. These results may aid in future research on the mechanism of the light-induced promoters and their application in plant biotechnology and plant genetics.Multiple intracranial aneurysms: rupture pattern and clinical outcome.
The optimal treatment for multiple aneurysms is still debated. We analyzed retrospectively the outcome of patients who underwent aneurysm treatment at the authors’ institution and whose aneurysms were treated either with microsurgical clipping or endovascular coiling to determine which type of treatment was associated with a better prognosis. Patients treated for multiple intracranial aneurysms from January 1995 through July 2004 were identified from a hospital neurosurgical database. The authors assessed clinical outcome according to aneurysm treatment and aneurysm location and size as well as intraoperative and postoperative complications. Outcome was assessed at discharge and at follow-up examination after 6 months and 1 year. The authors identified 84 patients who were treated for intracranial aneurysms: 43 patients (51%) were treated for multiple aneurysms (group 1), and 41 patients (49%) were treated for single aneurysms (group 2). Multiple aneurysms were found in 5.6% of patients with isolated aneurysms.
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